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In vivo activities of ceftriaxone and vancomycin

Posted: Thu 9 Apr 2009 19:19
by cave76
In vivo activities of ceftriaxone and vancomycin against Borrelia spp. in the mouse brain and other sites.
Kazragis RJ, Dever LL, Jorgensen JH, Barbour AG.

Department of Medicine (Infectious Diseases), University of Texas Health Science Center at San Antonio 78284, USA.

Borrelia burgdorferi, the agent of Lyme disease, and B. turicatae, a neurotropic agent of relapsing fever, are susceptible to vancomycin in vitro, with an MIC of 0.5 microgram/ml. To determine the activity of vancomycin in vivo, particularly in the brain, we infected adult immunocompetent BALB/c and immunodeficient CB-17 scid mice with B. burgdorferi or B. turicatae. The mice were then treated with vancomycin, ceftriaxone as a positive control, or normal saline as a negative control. The effectiveness of treatment was assessed by cultures of blood and brain and other tissues. Ceftriaxone at a dose of 25 mg/kg of body weight administered every 12 h for 7 to 10 days eliminated cultivable B. burgdorferi or B. turicatae from all BALB/c or scid mice in the study. Vancomycin at 30 mg/kg administered every 12 h was effective in eliminating infection from immunodeficient mice if treatment was started within 3 days of the onset of infection. If treatment with vancomycin was delayed for 7 days or more, vancomycin failed to eradicate infection with B. burgdorferi or B. turicatae from immunodeficient mice. The failure of vancomycin in eradicating established infections in immunodeficient mice was associated with the persistence of viable spirochetes in the brain during antibiotic treatment.

PMID: 8913478 [PubMed - indexed for MEDLINE]

PMCID: PMC163589

Re: In vivo activities of ceftriaxone and vancomycin

Posted: Fri 10 Apr 2009 1:15
by Joe Ham
This 1996 paper about the dynamics of ceftriaxone and vancomycin was discussed in some detail in a thread about a year ago:
http://www.lymeneteurope.org/forum/view ... 9890#p9890
There is a link to the full version in that post.

You may remember it as the one in which you asked about the early invasion of the brain.

I find that an easy way to search for previous postings so as to avoid needless duplication is to search for some more or less unique word that is used in the paper. In many cases the name of the lead author does nicely. For example in this case 'Kazragis' will do it and finds the paper even tho the title of the paper is not reflected in the title of the thread.

Re: In vivo activities of ceftriaxone and vancomycin

Posted: Fri 10 Apr 2009 3:48
by Fin24
and if like MOST of us, what if we dont recall the authors' names????

hey Joe seriously now I AM worried about your labile emotional status i.e. crankiness and other than declining physical health I cant imagine any other reasons, so YES I am now quite concerned about you--is everything allright??

Re: In vivo activities of ceftriaxone and vancomycin

Posted: Fri 10 Apr 2009 16:05
by cave76
I usually write the name of the lead author down on a Post-It note.

[personal non contributory, non substantial commentary removed]