Prof Morten M. Laanes paper is out - detection method

Topics with information and discussion about published studies related to Lyme disease and other tick-borne diseases.
Post Reply
Trillis
Posts: 7
Joined: Fri 22 Jun 2012 22:02

Prof Morten M. Laanes paper is out - detection method

Post by Trillis » Wed 6 Mar 2013 7:08

Just saw that Prof Morten M. Laane's paper is out today: "A simple method for the detection of live Borrelia spirochaetes in human blood using classical microscopy techniques"

http://www.biomedicalreports.org/index. ... h%5B%5D=98

User avatar
inmacdonald
Posts: 976
Joined: Fri 13 Jan 2012 22:32

Re: Prof Morten M. Laanes paper is out - detection method

Post by inmacdonald » Wed 6 Mar 2013 13:34

Prof. Morton Laane has extensive experience in all aspects of Academic microscopy
and is a Fellow of the Royal Academy of Microscopists. He has worked to perfect
the Live blood studies for presence of Borrelia from the blood of human patients
with Lyme Borreliosis.'

His atlas of Live blood images of various forms of Cystic borrelia, spiral forms,
intracellular forms add a considerable dimension to the recently published
paper on Isolation of Borrelia from the blood of 72 patients with Chronic LB
from the United States by Advanced Labs.

The full text PDF is freely available at the base of the Abstract posted above,
I recommend that all persons read the full text, and study the abstracts.
Best,
Alan

Bagge
Posts: 596
Joined: Wed 19 Oct 2011 19:49

Re: Prof Morten M. Laanes paper is out - detection method

Post by Bagge » Wed 6 Mar 2013 16:18

inmacdonald wrote:Prof. Morton Laane has extensive experience in all aspects of Academic microscopy
and is a Fellow of the Royal Academy of Microscopists. He has worked to perfect
the Live blood studies for presence of Borrelia from the blood of human patients
with Lyme Borreliosis.'

His atlas of Live blood images of various forms of Cystic borrelia, spiral forms,
intracellular forms add a considerable dimension to the recently published
paper on Isolation of Borrelia from the blood of 72 patients with Chronic LB
from the United States by Advanced Labs.

The full text PDF is freely available at the base of the Abstract posted above,
I recommend that all persons read the full text, and study the abstracts.
Best,
Alan

References for the Laane article are as follows
, (Highlights added)

http://www.biomedicalreports.org/index. ... &path[]=98
References

References

Samuels, S. D.; Radolf, J. D. (eds), Borrelia. Molecular biology, host interaction and pathogenesis. Norfolk, Caister Academic Press. 547pp. (2010). ISBN 978-1-904455-58-5

Gray, J. S.; Kahl, O.; Lane, R. S.; Stanek, G. (eds), Lyme borreliosis. Biology,

epidemiology and control. New York, CABI Publishing, 347pp. (2002) ISBN 0851996329

Stricker, R. B.; Johnson, L., Lyme disease: the next decade. Infection and Drug Resistance 4 (2011), pp. 1-9. DOI: http:/dx.doi.org/10.2147/IDR.S15653

Burgdorfer, W., Lecture 12th Internat. Congr. On Lyme disease and other spirochetal and tick-borne disorders (1999). http://en.wikipedia.org/wiki/Willy_Burgdorfer

Hindle, E., On the life cycle of Spirochaeta gallinarum. Parasitology IV 1912, 463-477 (http://printfu.org/borrelia+burgdorferi+life+cycle).

Ferguson, J., Cure unwanted? Exploring the chronic Lyme disease controversy and why conflicts of interest in practice guidelines may be guidelines guiding us down the wrong path. American Journal of Law and Medicine 2012, 38, 196-224 .

Laane
M. M., Ein Einfaches Mikroskopiesystem für Zeitrafferaufnahmen lebender Zellen. Mikrokosmos (Stuttgart) 2006, 95, 310-6. (A simple microscopical system for time-lapse records of living cells). In German

Laane, M. M.; Lie, T., Moderne mikroskopi med enkle metoder. Unipub forlag, Oslo. (2007), pp. 95-101. ISBN 978-82-7477-281-6. (Modern microscopy with simple methods). In Norwegian

Laane, M. M., The use of webcams in microscopy. “InFocus” Magazine, - The Proceedings of The Royal Microscopical Society, Oxford. Issue 2 (2007), pp. 42-54

http://www.rms.org.uk/OneStopCMS/Core/C ... 588aa7dd90

Laane, M. M.; Haugli, F. B., Illustrated guide to phase-contrast microscopy of nuclear events during mitosis and meiosis. In: H.C. Aldrich, J.W. Daniel (eds). Cell biology of Physarum and Didymium. New York, Academic Press Inc., Vol. 2 (1982), pp. 265-276.

ISBN 0-12-049602-X

Yu, L. W., Kjerneorganisering i Parabasalia. Et bidrag til forståelsen av mitosemekanismen som dobbeltsystem. (Nuclear organization in Parabasalids. A contribution to the understanding of the double nature of the mitotic mechanism). M.Sc. Thesis (supervisor M.M. Laane). University of Oslo, Norway. (2000)

Laane, M. M., YouTube video “SuperMikroskop” Borrelia live in human blood. (2011). http://www.youtube.com/watch?v=YxSHL9xG ... re=related

Laane, M. M.; Mysterud, I.; Longva, O.; Schumacher, T., Borrelia og Lyme-borreliose,- morfologiske studier av en farlig spirochet. Biolog 2009, 27, (2), 30-45. (Borrelia and Lyme borreliosis, - morphological studies of a dangerous spirochaete). In Norwegian. http://www.bio.ekanal.no/bio/vedlegg/borrelia.pdf

Laane, M. M.; Olsson, C. C.; Mysterud, I.; Longva, O.; Schumacher, T., Flått og hjortelusflue - viktige sykdomsvektorer under spredning i norsk natur. Biolog 2010, 28, (3-4), 70-85. (Tick and deerked – important disease vectors spreading in Norwegian ecosystems). In Norwegian.

http://www.borrelia-tbe.se/filer/2011/04/Fltt_og_hjortelusflue_frn_tidsskriften_Biolog.pdf

Sens, P.; Gov, N., Force balance and membrane shedding at the red blood cell surface. Physical Review letters 2007, 98 018102, 1-4.

Margulis, L. J.; Ashen, B.; Sole, M.; Gurrero, R., Composite, large spirochetes from microbial mats: spirochetes structure review. Proc Natl Acad Sci USA 1993, 90, 6966-70.

Brorson, Ø.; Brorson, S.-H.; Schytes, J.; McAllister, J.; Wier, A.; Margulis, L., Destruction of the spirochaete Borrelia burgdorferi round-body propagules (RBs) by the antibiotic Tigeocycline. PNAS 2009, 106, (44), 18656-61.

Brorson, Ø.; Brorson, S.-H., A rapid method for generating cystic forms of Borrelia burgdorferi, and their reversal to mobile spirochetes. APMIS 1998, 106, (12), 1131-41 .

The Lyme Info Net, see especially: Morphological transformation in Borrelia and other spirochetes: Observations of round forms and blebs 1905-2010, see also Survival in adverse conditions. http://www.lymeinfo.net/lymefiles.html

Brorson, Ø.; Brorson, S.-H., Transformation of cystic forms of Borrelia burgdorferi to normal, mobile spirochetes. Infection 1997, 25, (4), 240-6.

Bozsik, B., YouTube video: Dark-field microscopy, DualDur (2009). http://www.youtube.com/watch?v=AiwRTu9zg5k

Moriarty, T. J.; Norman, M. U.; Colarusso, P.; Bankhead, T.; Kubes, P.; Chaconas, G., Real-time high-resolution 3D imaging of the Lyme disease spirochete adhering to and escaping from the vasculature of a living host. PLOS Pathogens 2008, 4, (6), e1000090. doi: 10.1371/journal.ppat.1000090.

Pohlod, D. J.; Mattman, L. H.; Tunsdall, L., Structures suggesting cell-wall deficient forms detected in circulating erythrocytes by fluorochrome staining. Applied Microbiology 1972, 23, 262-7.

Mattman, L., Lecture at Saginow, May 6th. 10 Ann. Int. Conf. NIH Bethesda. MD. (1997)

Badon, S. J.; Fister, R. D.; Cable, R. G., Survival of Borrelia burgdorferi in blood products. Transfusion 1989, 29, (7), 581-3.

Nadelmann, R. B.; Sherer, C.; Mack, L.; Pavia, C. S.; Wormser, G. P., Survival of Borrelia burgdorferi in human blood stored under blood banking conditions. Transfusion 1990, 30, (4), 298-301.

Margulis, L., (pers. comm.) letter to Dr. Todd LePine (2011).

Silvie, O.; Mota, M. M.; Matuschewski, K.; Prudencio, M., Interactions of the malaria parasite and its mammalian host. Current Opinion in Microbiology 2008, 11, 1-8.

MacDonald, A.
, A life-cycle for Borrelia spirochetes? Medical Hypotheses 2006, 67, (4), 810-8. .http://www.theoneclickgroup.co.uk/docum ... chetes.htm

MacDonald, A.
, Segmentation in the Borrelia genome. Genetic implications. (2012). http://lymeneteurope.org/forum/viewtopic.php?f=5&t=3686

YouTube videos, Wikipedia articles, and comments and images posted on Internet-based anonymous user support forums where 'you too can be a Lyme disease expert' do not make for an impressive list of scientific references.
.

X-member
Posts: 4092
Joined: Mon 30 Jul 2007 18:18

Re: Prof Morten M. Laanes paper is out - detection method

Post by X-member » Fri 12 Apr 2013 0:28

From fulltext:
Laboratory facilities and security measures

As a working environment a basement laboratory with no vibrations is important.
What do hv808ct say about that? 8-)

X-member
Posts: 4092
Joined: Mon 30 Jul 2007 18:18

Re: Prof Morten M. Laanes paper is out - detection method

Post by X-member » Wed 5 Jun 2013 20:48

Classic microscopy reveals borrelia bacteria

http://phys.org/news/2013-06-classic-mi ... teria.html
LARS MONSEN'S blood: Among the many sick people who have come to the two biologists at Blindern with samples of their blood is Lars Monsen. His blood is among the most contaminated the researchers have seen. It is full of bacteria and other contaminants. The Norwegian wilderness expert and expedition leader has a severe infection which is partly due to Borrelia. Credit: Morten Laane

X-member
Posts: 4092
Joined: Mon 30 Jul 2007 18:18

Re: Prof Morten M. Laanes paper is out - detection method

Post by X-member » Tue 25 Jun 2013 17:02

Relative Risk:

"Could this be the worst Lyme disease paper ever published?

Biological and Biomedical Reports, 2013, 3(1), 15-28
A simple method for the detection of live Borrelia spirochaetes in human blood using classical microscopy techniques. Morten M. Laane, Ivar Mysterud.
"

http://relative-risk.blogspot.se/2013/0 ... paper.html

A quote (from one comment):
Incidentally, there is no evidence that Borrelia reside in erythrocytes, despite Carina's delusions to the contrary.
A quote (from the text under a picture in Lida H Mattmans book: Cell Wall Deficient Forms Stealth Pathogens, 3rd edition):
Erythrocytes are filled with Lyme disease antigen as shown by fluorescent antibody.
Is it only the Lyme antigen that is found in the erythrocytes?

And, what is it that we can see in the pictures that Lida H Mattman show us on the link (YouTube) below, then?

Lida Mattman at the Autoimmunity Research Foundation's Chicago Conference

http://www.youtube.com/watch?v=WozrCFW0mRM

Camp Other
Posts: 996
Joined: Wed 2 Mar 2011 4:32
Contact:

Re: Prof Morten M. Laanes paper is out - detection method

Post by Camp Other » Tue 25 Jun 2013 20:18

Carina wrote:Relative Risk:

"Could this be the worst Lyme disease paper ever published?

Biological and Biomedical Reports, 2013, 3(1), 15-28
A simple method for the detection of live Borrelia spirochaetes in human blood using classical microscopy techniques. Morten M. Laane, Ivar Mysterud.
"

http://relative-risk.blogspot.se/2013/0 ... paper.html

A quote (from one comment):
Incidentally, there is no evidence that Borrelia reside in erythrocytes, despite Carina's delusions to the contrary.
A quote (from the text under a picture in Lida H Mattmans book: Cell Wall Deficient Forms Stealth Pathogens, 3rd edition):
Erythrocytes are filled with Lyme disease antigen as shown by fluorescent antibody.
Is it only the Lyme antigen that is found in the erythrocytes?

And, what is it that we can see in the pictures that Lida H Mattman show us on the link (YouTube) below, then?

Lida Mattman at the Autoimmunity Research Foundation's Chicago Conference

http://www.youtube.com/watch?v=WozrCFW0mRM
I would have liked to have seen additional information in Laane's paper.

But whoever that commenter is, they are incorrect, and they need not refer to Lida Mattman's work alone. There are other researchers who have discovered Borrelia inside erythrocytes (red blood cells).

Generally, relapsing fever spirochetes have invaded erythrocytes. I don't know who has found them in Borrelia burgdorferi, and if so, this is something which needs to be confirmed and reproduced so the scientific and medical community is aware of it.

Refer to:

The Spirochete Borrelia crocidurae Causes Erythrocyte Rosetting during Relapsing Fever. (1998)
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC107974/

ABSTRACT
Several species of the genus Borrelia exhibit antigenic variation of variable major proteins on their surface during relapsing fever. We have investigated the African relapsing fever species Borrelia crocidurae during infections in mice and compared it with the thoroughly studied North American species Borrelia hermsii. A major difference between the two species is that B. crocidurae can bind and become completely covered with erythrocytes. In addition, B. crocidurae causes a prolonged spirochetemia which coincides with a delayed appearance of antiborrelial antibodies. We show that the antibody response against an unrelated antigen is not delayed and that antibiotic treatment, which dissociates rosettes and inhibits the spirochetes, also leads to an early antibody response. Taken together, the erythrocyte aggregation and prolonged spirochetemia hint at a new mode of immune evasion where erythrocyte-covered spirochetes may avoid contact with the phagocytic cells and B cells of the immune system, thereby delaying the onset of a specific immune response.

Erythrocyte-aggregating relapsing fever spirochete Borrelia crocidurae induces formation of microemboli.
http://www.ncbi.nlm.nih.gov/pubmed/10558950

Abstract
The African relapsing fever spirochete Borrelia crocidurae forms aggregates with erythrocytes, resulting in a delayed immune response. Mice were infected with B. crocidurae and monitored during 50 days after infection. Spirochetes were observed extravascularly at day 2 after infection. Two days later, inflammatory responses, cell death, and tissue damage were evident. The pathologic responses in lungs and kidneys were similar, whereas the symptoms in the brains were delayed, with a less pronounced inflammatory response. Microemboli were found in the blood vessels, possibly a result of the erythrocyte aggregation. The B. crocidurae invasion emerged more rapidly than has been described for Lyme disease-causing Borrelia species. In addition to erythrocyte rosetting, the presence of extravascular B. crocidurae indicates a novel route for these bacteria to propagate and cause damage in the mammalian host. The histopathologic findings in this study may explain the clinical manifestations of human relapsing fever.

Okay, those papers are a little old. Let's go for something more recent:

Relapsing fever Borrelia binds to neolacto glycans and mediates rosetting of human erythrocytes. (2009)
http://www.ncbi.nlm.nih.gov/pubmed/19884498

Abstract
A hallmark of acute relapsing fever borreliosis is severe bacteremia. Some Borrelia species, such as B. duttonii and B. crocidurae, associate with erythrocytes and induce aggregation recognized as erythrocyte rosetting. Erythrocyte rosettes contribute to disease severity by increased tissue invasiveness (such as invasion of CNS and encephalitis), hemorrhaging, and reduced blood flow in affected microcapillaries. Here we report that relapsing fever Borrelia binds to neolacto (Galbeta4GlcNAcbeta3Galbeta4Glcbeta1)-carrying glycoconjugates that are present on human erythrocytes. This interaction is of low affinity but is compensated for by the multivalency of neo-lacto-oligosaccharides on the erythrocyte cell surface. Hence, the protein-carbohydrate interaction is dependent on multivalent neolacto-glycans to mediate binding.

Questions I ask after reading all this:

1) Can "Lyme disease" Borrelia spirochetes differ enough from strain to strain including ones in which we are uncertain about their pathogenicity - that some of them may very well invade erythrocytes?

2) Could North American and Eurasian relapsing fever spirochetes (B. miyamotoi for one) also form erythrocyte aggregations?

3) How many studies on this phenomenon have been conducted on other mammals outside of mice, including non-human primates and humans?

X-member
Posts: 4092
Joined: Mon 30 Jul 2007 18:18

Re: Prof Morten M. Laanes paper is out - detection method

Post by X-member » Thu 27 Jun 2013 19:48

Thank you for your help CO!

Let me see if I understood this correct.

Since Borrelia recurrentis and Borrelia miyamotoi (and so on) also are microaerophilic, the person that wrote the comment (in RR:s blog) must be wrong?

Pandora
Posts: 252
Joined: Tue 20 Mar 2012 14:58

Re: Prof Morten M. Laanes paper is out - detection method

Post by Pandora » Sun 30 Jun 2013 21:15

Why would anyone peruse RR's blog? He is nothing more than a psychotic obstructionist of the science.

X-member
Posts: 4092
Joined: Mon 30 Jul 2007 18:18

Re: Prof Morten M. Laanes paper is out - detection method

Post by X-member » Fri 13 Sep 2013 19:03

CspA from Borrelia burgdorferi Inhibits the Terminal Complement Pathway

http://mbio.asm.org/content/4/4/e00481-13.short


A quote:
IMPORTANCE The present study defines a new mechanism by which the pathogenic bacterium Borrelia burgdorferi controls the terminal complement pathway of the human host to survive in human serum.

Post Reply