Info about Lyme round bodies (cyst form)

General or non-medical topics with information and discussion related to Lyme disease and other tick-borne diseases.
X-member
Posts: 3954
Joined: Mon 30 Jul 2007 18:18

Re: Info about Lyme round bodies (cyst form)

Post by X-member » Wed 25 Jan 2012 0:50

From LymeRICK:

"Spirochetes and their cyst / granule / L-form"

http://lymerick.net/Spirochetal-cysts.htm

X-member
Posts: 3954
Joined: Mon 30 Jul 2007 18:18

Re: Info about Lyme round bodies (cyst form)

Post by X-member » Wed 25 Jan 2012 15:14

What is inside a cyst/round body?

Article from Norway:

"Borrelia og Lyme-borreliose
morfologiske studier av en farlig spirochet"

("Borrelia and Lyme borreliosis
morphological studies of a dangerous spirochet")

http://www.bio.ekanal.no/bio/vedlegg/borrelia.pdf

Look at the pictures at page 34/5.

If you need a translation, use google translate!

Not all the pictures in this article are on borrelia!

Camp Other
Posts: 996
Joined: Wed 2 Mar 2011 4:32
Contact:

Re: Info about Lyme round bodies (cyst form)

Post by Camp Other » Wed 25 Jan 2012 18:39

What I want to know is why it is I never hear or see any new papers discussing this form of Borrelia, gemma, as displayed in this paper:

Biology of Borrelia species.
A G Barbour and S F Hayes
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC373079/

Specifically, refer to this image:
http://www.ncbi.nlm.nih.gov/pmc/article ... 79/?page=7

If you download the .pdf, the image quality is much better. Or, actually, try this direct link to image only and not text: http://www.ncbi.nlm.nih.gov/pmc/article ... figure/F4/

User avatar
inmacdonald
Posts: 976
Joined: Fri 13 Jan 2012 22:32

Re: Info about Lyme round bodies (cyst form)

Post by inmacdonald » Wed 25 Jan 2012 23:25

+

Dear Camp.

Gemma (e) have a fairly respectable list of citations in [gemmae spirochetes] Google.
The term was a favorite of Dr Burgdorfer and his electron microscopist Fred Hayes.
The term is found on page 12 of my bibliography of cysts and adverse conditions
under the heading cysts / reproduction.
Dr Edward Delamater and Dr Greta Tyson among others, used the term in their papers.
The current group of investigators who are actively pursuing cystic forms
of borrelia have not chosen to write the word "gemmae" in their papers, not because
there is anything intrinsically wrong with gemma but perhaps to favor" Cyst,or cystic"
as more general descriptors.

In my view, one parameter which sets gemmae apart from other possible cystic morphologies
is the appearance of a "sphere loaded with granular forms which coexist with remnants of
the protoplasmic cell wall invested cylinder" .

I attach not one but two images of gemmae from the bibliographies
which I have posted earlier.
Attachments
gemmae number 2.jpg
Gemma number 2
gemmae number 2.jpg (16.98 KiB) Viewed 6164 times
gemmae.jpg
Gemma number 1
gemmae.jpg (35.49 KiB) Viewed 6164 times

User avatar
inmacdonald
Posts: 976
Joined: Fri 13 Jan 2012 22:32

Re: Info about Lyme round bodies (cyst form)

Post by inmacdonald » Thu 26 Jan 2012 0:00

Bacteriophages in the Ixodes dammini spirochete ..[[.Now renamed Ixodes..various species....]]

http://jb.asm.org/content/154/3/1436.full.pdf


I always wonder about the idea that the site of attachment ot the bacteriophage

Stays the same in figures 1 and 2, but when the membrane is "cytolyzed" (Figure 2)
it appears that it (the spirochetal cell) is turned "inside out", because the site of bacteriophage attachment
remains the same, but the "polarity" of the phages is reversed.( ie the bacteriophage heads now point
towards the interior region in Fig 2 -- contrasted with the heads pointing to the exterior in fig 1.)
Is this a possible clue to what might be going on in the process of cyst formation....???
DOES THE CELL OUTER SURFACE TURN INSIDE OUT? if it does turn inside out,
does the organism present a different set of antigens to the body??
?

This is an intriguing study, which to my knowledge, has never been repeated.
Regrettably, the budget for electron microscopic study, is virtually non existent
in many labs which used to do such excellent work.

Alan B. MacDonald, MD
Attachments
BACTERIOPHAGES INSIDE THE PLASMA MEMBRANE.jpg
bacteriophages attached to the INSIDE of the membrane
BACTERIOPHAGES INSIDE THE PLASMA MEMBRANE.jpg (106.41 KiB) Viewed 6152 times
Bacteriophages outside the plasma membrane.jpg
bacteriophages attached to the OUTSIDE of the membrane
Bacteriophages outside the plasma membrane.jpg (62.24 KiB) Viewed 6152 times

User avatar
inmacdonald
Posts: 976
Joined: Fri 13 Jan 2012 22:32

Re: Info about Lyme round bodies (cyst form)

Post by inmacdonald » Thu 26 Jan 2012 0:03

Note; CORRECTION
FIGURE 1 heads point Inside
Figure 2 heads point outside

X-member
Posts: 3954
Joined: Mon 30 Jul 2007 18:18

Re: Info about Lyme round bodies (cyst form)

Post by X-member » Thu 26 Jan 2012 14:37

Thank you, Alan B MacDonald, MD!

In the video below on YouTube you can see a gemma (e) (in blood) after about 4,15 min:

"Dr Bozsik - Dark Field Microscopy DualDur (microscopie pe camp intunecat) - Borrelia Burgdorferi"

http://www.youtube.com/watch?v=AiwRTu9zg5k

User avatar
inmacdonald
Posts: 976
Joined: Fri 13 Jan 2012 22:32

Re: Info about Lyme round bodies (cyst form)

Post by inmacdonald » Mon 30 Jan 2012 15:26

Dynamic images ofBorrelia Transition from Spiral (vegetative) to round (Cystic) forms
Darkfield images from the B31 reference strain - in vitro culture
Attachments
Borrelia Transition Vegetative form to cystic form.jpg
Borrelia burgdorferi in transition to cystic form- Darkfield microscopy 1000x magnification
Borrelia Transition Vegetative form to cystic form.jpg (43.79 KiB) Viewed 6089 times

User avatar
inmacdonald
Posts: 976
Joined: Fri 13 Jan 2012 22:32

Re: Info about Lyme round bodies (cyst form)

Post by inmacdonald » Mon 30 Jan 2012 15:41

Dynamic Images Borrelia Cysts and their Spiral (vegetatve) precursors in Darkfield microscopy .
Reference strain B31 Borrelia burgdorferi in BSK culture.
Attachments
Borrelia Transition Vegetative form to cystic form.jpg
Coexistent Borrelia Cyst form and spiral (vegetative) form which is partially "encysted"
Borrelia Transition Vegetative form to cystic form.jpg (43.79 KiB) Viewed 6087 times
Cyst in Transition.jpg
Cyst of Borrelia with "rolled up" spiral form" and a "tail" which may be on its way to the inside ....or ...emerging from the interioir to the "outside"
Cyst in Transition.jpg (69.83 KiB) Viewed 6087 times

X-member
Posts: 3954
Joined: Mon 30 Jul 2007 18:18

Re: Info about Lyme round bodies (cyst form)

Post by X-member » Tue 31 Jan 2012 19:03

Thank you! Great pictures! 8-)

Post Reply